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KMID : 0903520070500030238
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2007 Volume.50 No. 3 p.238 ~ p.243
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Analysis of the Limonoid Contents of Dangyuja (Citrus spp.) by Liquid Chromatography-mass Spectrometry (LC-MS)
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Boo Kyung-Hwan
Kim Ha-Na
Riu Key-Zung
Kim Youn-Woo
Cho Moon-Jae
Kim So-Mi
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Abstract
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The contents of limonin, nomilin and limonin glucoside in different tissues of dangyuja (Citrus spp.) were measured during fruit growth and maturation by liquid chromatography-mass spectrometry (LC-MS). Both nomilin and limonin contents increased from June, peaked in October and in December, respectively, and then decreased afterwards. In contrast, the content of limonin glucoside increased from October and remained at high levels throughout the maturation. The contents of limonin, nomilin and limonin glucoside in peel decreased gradually throughout the growth and the trends of changes were similar to each other. However, all three limonoid contents in juice sac increased dramatically at the end of fruit maturation. The highest concentration of limonin and nomilin was observed in seed, followed by segment membrane, whereas the highest limonin glucoside concentration was observed in segment membrane, followed by juice sac.
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KEYWORD
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citrus, dangyuja, LC-MS, limonin, limonin glucoside, nomilin
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